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Device

Part:BBa_K651000:Design

Designed by: Sung won Lim, Hannah Landes, Balvir Kunar   Group: iGEM11_NYC_Software   (2011-09-26)


RecA promoter with RFP coding device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 747
    Illegal AgeI site found at 859
    Illegal AgeI site found at 1695
    Illegal AgeI site found at 2910
    Illegal AgeI site found at 3022
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Automated biobricking programs coupled with ability to look up genbank and other web based DNA databases speed up the ease and speed of compositing simple systems significantly.


Source

recA promoter was lifted via colony PCR from JM 109 strain of E.Coli with RFC 23 overhangs. RBS and RFP generator are existing biobricks from the registry. (B0034 and mRFP1 E1010)

References